B.Sc. Botany Semester 6 CORE Course; Course Name- Plant tissue culture and its biotechnological applications – BOT6; Course Duration- Theory – 60 Hrs., Practical – 30 Hrs.; Instructor: KVM



Tissue culture involves growing plants from very small sections (sometimes microscopic) in a laboratory. It is a propagation method which is being increasingly used. Tissue culture is not appropriate for many plants, but for others such as orchids, some indoor plants and in particular, many new plant varieties, it is a very popular propagation method. To be more particular, this course is divided into quadrants that are consequently divided into lessons each lesson covering a topic in substantial details.


· Quadrant 1 one covers the History of Plant Tissue Culture

· Quadrant 2 two deals with Methodology of Tissue culture.

· Quadrant 3 deals the Applied Plant Tissue Culture .

· Quadrant 4 covers the production of secondary metabolotes


Plants have been conventionally propagated by seed and vegetative methods. Seeds are generally heterozygous and cause undesirable variability in the off-springs. Moreover, methods of vegetative propagation are not available for all the plant species. Further, these methods, wherever available, are slow and able to produce a limited number of propagules. Plant Tissue Culture has become an important alternative where any plant species can be cloned and a large number of genetically uniform disease-free plants can be obtained within a short period of time. The technique enables production of plants in a small laboratory space round the year, independent of season. The controlled physico-chemical culture environment ensures uniform growth and optimum field performance of the plants. The technique has been widely applied for large-scale production of quality plants including fruit plants, ornamentals, plantation crops, tree species, spices and condiments. The demand for tissue culture derived plants has been growing exponentially across the globe. A large number of tissue culture based industries have been engaged in commercial production of selected plant species in India and abroad. There is a huge demand for trained manpower in this sector. The course being offered will train manpower suited to the needs of the industry so that the trained personnel become employable. In addition, the course will also provide advanced training to become an entrepreneur in the said area.


The students will gain overall knowledge of:

· To train manpower suited to the needs of Plant Tissue Culture Industry and Research centers.

· To impart advanced training in plant tissue culture technology to develop entrepreneurship skills.

The online version of the course will provide you with additional resources such as post lecture assignments (with bonus points), online activities, quizzes and computer based exams (both models and past years exams). Finally, the most important advantage of this online version is having the chance to directly interact with the course material (posting comments and suggesting ideas) and with the course coordinator (posting questions and debating answers).

As always we are looking forward to hearing your comments and ideas about the course. Please feel free to contact the course author, Dr. Madhusudhan kalaiahgari V, through email at [email protected]

We had enough talk, it is time to go to the real world and start your journey inside the cell. I hope you enjoy it!

About Syllabus

· Unit 1 – History of plant tissue culture

· Unit 1-Post-assessment

· Unit 2 – Plant Nutrients

· Unit 2-Post-assessment

· Unit 3 – The Laboratory

· Unit 3-Post-assessment

· Unit 4 – Media Preparation and types

· Unit 4-Post-assessment

· Unit 5 – Organ culture

· Unit 5-Post-assessment

· Unit 6 – Embryo culture

· Unit-6-Post-assessment

· Unit 7 – Endosperm culture

· Unit 7-Post-assessment

· Unit 8 – Callus culture

· Unit 9 – Micropropogation

· Unit 9-Post-assessment

· Unit 10 – Applications of Tissue culture

· Unit 10-Post-assessment

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